Phytochemical and Biological Studies of Butea Frondosa Roxb. Leaves Growing in Egypt (2025)

Evaluation of Total Phenolic Content and Investigation of Potent Flavonoid Compound Isolated from Butea frondosa Roxb

2020

In this research work, the stem bark of ButeafrondosaRoxb., Myanmar name Pauk, was selected for chemical analysis. This samplewas collected from Amarapura Township, Mandalay Region, Myanmar. The aim of this research is to get more interesting and bioactive compounds from this versatile medicinal plant. Firstly, the preliminary phytochemical test of this selected plant was carried out. Moreover, the total phenols of selected sample were extracted with methanol and distilled water. This extract was checked for qualitative test of phenols. In addition, total phenolic content of selected sample was evaluated by the FolinCiocalteau reagent using UV spectrophotometer at 765 nm. The total phenolic content ofselected sample was found to be15.40.023 mg gallic acid equivalents (GAE) per g dry weight.Furthermore, the potent pure compound was isolated by Column Chromatography and HPLC methods atMeijo University, Nagoya, Japan. The structure of isolated compound was elucidated as flavonoid by a...

Physico-chemical evaluation and phytochemical potential of a medicinal herb: Butea frondosa Koen. Ex Roxb (leaves

The present work attempts to evaluate the physicochemical and preliminary phytochemical studies on the leaves of Butea frondosa Koen. Ex Roxb, family Fabaceae. Methods: The herbal standardization was carried out on the basis of organoleptic properties, physical characteristics, and physico-chemical properties. Physicochemical parameters including ash values, extractive values, loss on drying, foreign matter were evaluated. Results: Macroscopically leaves were observed to be compound. Lateral leaflets were obliquely ovate, obtuse, round at apex. Its texture was fairly tough. Microscopical leaves have single layered upper and lower epidermis, covered with thick cuticle. Xylem and phloem are arranged in ring. Calcium oxylate crystal were scattered throughout the cells. The total ash, acid insoluble ash and water soluble ash were found to be 10.16%, 2.83%, and 5.16% w/w respectively. Petroleum ether, Chloroform, ethanol and water soluble extractive values (hot)were 2.94%, 3.08%, 5.06%, 10.61%w/w respectively. The pH of 1% and 10% aqueous solution was found to be 6.06 and 5.76 respectively. Preliminary phytochemical screening showed the presence of sterols, tannins, flavonoids, amino acids, glycosides, phenolic compounds, carbohydrates, saponins and alkaloids. Thin layer chromatographic studies also had been done on ethanolic and aqueous extracts. Conclusion: These studies provided referential information for correct identification and standardization of this plant material.

Estrogenic and Anti-Estrogenic Activities of the Thai Traditional Herb,Butea superbaRoxb

Bioscience, Biotechnology, and Biochemistry, 2010

This study evaluated the estrogenic and antiestrogenic activities of native and in vitro hepatic metabolized tuberous extracts of wild Butea superba collected from 23 out of the 76 provinces in Thailand by yeast estrogen screening (YES). The YES screen used consisted of the human estrogen receptors hER and hER and the human transcriptional intermediary factor 2 or human steroid receptor coactivator 1, respectively, together with the-galactosidase expression cassette as the reporter. The relative potency, effectiveness and relative inductive efficiency were evaluated by determining the-galactosidase activity (EC 50) of each tuberous extract in relation to that induced by 17-estradiol. Six pure compounds isolated from B. superba were tested in parallel and exhibited a maximum relative potency compared to 17-estradiol of 15.5% and 5.27% in the respective hER and hER assays. Eighteen and seventeen plant extracts were respectively found to interact with the hER and hER receptors in the YES assays with higher relative potency and relative inductive efficiency with hER than with hER. The selected plant extracts tested exhibited antiestrogenic activity. Coincubation with the rat liver S9 mixture also elevated the estrogenic potency of these plant extracts.

Estrogenic activities of ten medicinal herbs from the Middle East

2012

Traditional medicinal plants have long been recognized as remedies and important sources of treatment for developing countries. In the present study, we report on a detailed study to quantify the presence of five known phytoestrogens in 10 widely used herbs used in the Middle East. Surprisingly some of these plants were almost devoid of tested phytoestrogens, whereas others were very rich in known phytoestrogens. For example, Hibiscus sabdariffa was found to be the richest in quercetin and daidzein, whereas Cyperus conglomeratus had the highest concentrations of kaempferol and genistein. On the other hand, Salvadora persica was almost devoid of the screened phytoestrogens. Ethanolic extracts were further tested for their proliferative activities in cell-culture using estrogenresponsive breast cancer cell lines (MCF-7) and were found to fall into three distinct groups based on their estrogenic activities. The most potent herbal extract (O. vulgare) was further fractionated and the fractions were analyzed again for phytoestrogenic content (using high-performance liquid chromatography) and proliferative activity. Our results indicate that the proliferative activities of some of the extracts and fractions are not completely attributable to the phytoestrogens screened, thus it is likely that some of these plants may have other (perhaps yet unknown) phytoestrogens.

Isolation and identification of a flavone (quercetin) from Butea frondosa bark

Pharmaceutical Chemistry Journal, 2007

A flavone was isolated from the stem bark of Butea frondosa (Leguminosae). It was given a working name of BF-1 and characterized by m.p., 309 -311°C and an empirical formula of C 15 H 10 O 7 . On the basis of chemical and spectral evidence and upon comparison with the literature data, the isolated compound is identified for the first time as quercetin.

Estradiol-antagonistic activity of phenolic compounds from leguminous plants

Phytotherapy Research, 2008

Natural flavonoids are currently receiving much attention because of their estrogenic and antiestrogenic properties. Six isoflavones (isoprunetin, isoprunetin 7-O-β-d-glucopyranoside, isoprunetin 4′,7-di-O-β-d-glucopyranoside, genistein, genistein 7-O-β-d-glucopyranoside, daidzein), four flavones (luteolin, luteolin 7-O-β-d-glucopyranoside, luteolin 4′-O-β-d-glucopyranoside, licoflavone C), isolated from Genista morisii and G. ephedroides (two Leguminosae plants of the Mediterranean area) together with two structurally related pterocarpans, bitucarpin A and erybraedyn C, isolated from Bituminaria bituminosa (Leguminosae), were tested for the antagonist activity by a yeast based estrogen receptor assay (Saccharomyces cerevisiae RMY326 ER-ERE). Most compounds inhibited the estradiol-induced transcriptional activity in a concentration dependent manner. In particular, for the flavone luteolin 77% inhibition of the induced β-galactosidase activity was observed. Interestingly, licoflavone C exhibited a dose-dependent antagonistic activity at concentrations up to 10−4 m, but stimulated β-galactosidase expression at higher concentrations resulting in a U-shaped-like dose-response curve. Copyright © 2007 John Wiley & Sons, Ltd.

IN-VITRO PHYTOCHEMICAL SCREENING OF THE FLOWER EXTRACTS OF BUTEA MONOSPEMA (LAM.) TAUB

International Journal of Current Research, 2021

Aqueous and acetone extracts of the plant Butea monospema flowers of the family Leguminosae-Papilioneae.were prepared by adding 25 g of fresh flowers to 100 ml of these solvents. The constituents were extracted by soxhlet extractor for 24 h. After incubation, the extracts were collected and stored at 4°C.In the present study, all the seven groups of the important phytocompounds, viz., cardiac glycosides, steroids, alkaloids, flavonoids, terpenoids, tannins and saponins, were screened from the plant Butea monospema flowers. Their presence was confirmed through in vitro phytochemical screening of aqueous and acetone extracts of flower. The analysis for the same was carried out by qualitative tests for which the standard protocol was followed. The presence or absence of the phyto-constituents depended upon the solvent used and physiological property of the flowers. From these results, it can be concluded that the B. monosperma flower extracts may be used as broadspectrum bioactive agents after extensive investigation.

Oestrogenic properties of the ethanolic extract of Fernandoa adolfi friderici (Bignoniaceae) stem bark

Fernandoa adolfi friderici is used traditionally to treat menopause related symptoms. The effects of the ethanolic extract of this plant in ovariectomized (OVX) rats were investigated. 40 of 50 rats were OVX and separated into four groups of 10 each. 84 days after ovariectomy, animal groups were started on one of four treatments: ethanol/0.09% NaCl (control group), oestrogen, extracts of F. adolfi friderici (FA 150 and 300 mg/kg), respectively. 5 animals from each group were terminated after 14 and 28 days of treatment, respectively and vaginal smears, vaginal/uterine wall cytology, lipid profiles and bone mass and histology were studied. F. adolfi friderici treated OVX rats had superficial cells which were absent in vaginal smears of control animals. The former had improved vaginal epithelial thickness after 28 days of treatment (55.8±8.6 versus 13.2±0.8) compared to control animals. F. adolfi friderici extracts also improved serum triglyceride (27.2%/28.2% reduction versus 73% increase in controls) and high density lipoprotein (HDL) (0.78±0.01/0.98±0.09 g/L versus 0.6±0.05 g/L) levels while improving bone architecture and preventing bone resorption. F. adolfi friderici may be the source of a useful phytoestrogen for the complementary management of menopausal symptoms.

ANTIESTROGENIC AND ANTIANDROGENIC ACTIVITIES OF A HYDRO-ALCOHOLIC LEAF EXTRACT OF PENTADESMA BUTYRACEA (CLUSIACEAE).

Menopausal symptoms and hormone-related diseases such as breast and prostate cancers are serious problems of public health. The management of these diseases is extremely expensive for populations in developing countries with low outcomes. As alternative, medicinal plants are the most used for healthcare in these countries. Therefore, several preclinical studies are performed to screen phytoestrogens and phytoandrogens which can exhibit health benefits for management of these symptoms and hormone-related diseases. Pentadesma butyracea is a West African rainforest species used traditionally for management of reproductive disorders and treatment of breast pain. Thus, the aim of this study was to assess hormone-like activities of a hydro-alcoholic leaf extract of P. butyracea by in vitro and in vivo assays. Yeast estrogen/androgen screen were performed to evaluate (anti)estrogenic and (anti)androgenic activities of the extract. For the in vivo assay, Hershberger bioassay was performed in immature male Wistar rats. The weight of accessory glands was used for the evaluation of (anti)androgenic activities. Immature uterotrophic bioassay was used as in vivo assay for detection of possible (anti)estrogenic activities based on uterine, ovarian and adrenal weight. In the yeast estrogen/androgen screen, the extract exhibited antiestrogenic and antiandrogenic activities. In vivo assays confirmed that the extract exhibited antiandrogenic (p < 0.05) activity but did not have antiestrogenic activity. Overall, the extract did not exhibit estrogenic and androgenic properties, but showed antiestrogenic activity in vitro and antiandrogenic activities both in vitro and in vivo. These findings showed the extract can antagonized estrogenic activity and could act Selective Androgen Regulator Modulators. Therefore, it can have a great potential of application for regulation of sex hormone target tissues including management of hormone-related diseases. Nevertheless, subsequent studies are necessary to assess its safety in terms of breast and prostate cancer.

Exploration of phytochemical potential on flower of Butea monosperma

Journal of Pharmacognosy and Phytochemistry, 2019

Phytochemical exploration is an important step in the finding of bioactive composite present in medicinal flora. The Butea monosperma flower extracts and its solvent fractionates was subjected to preliminary phytochemical screening using standard phytochemical tests. The aim of the present study was to investigate the presence of phytochemicals. Soxhlet apparatus was used for the organic solvent extraction. These investigations revealed the presence of flavonoids, tannins, saponins, carbohydrates, terpenoids, alkaloids, proteins, quinones, phenols and glycosides in the flower of the plant extracts. The presence of various bioactive compounds confirms the application of B. Monosperma for various ailments by traditional practitioners. However, isolation of individual phytochemical constituents may proceed to find a novel drug.